| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.005332 |
| Chromosome: | chromosome 15 |
| Location: | 295850 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g635100 | (1 of 2) IPR000104//IPR010402 - Antifreeze protein, type I // CCT domain | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTCTCCCGCGCCGCCTGGCAACCTCAAACAGGTGCCTACATCTTTGAA |
| Internal bar code: | ACCATAAGCATAGTATATAATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 655 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGGCTGGATGCAAGGTTA |
| Suggested primer 2: | AACGACGCTTTTTGGGCTTC |