Insertion junction: CLIP2.005339_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.005339
Chromosome:chromosome 3
Location:4947643
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g179200 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCGAAGGTCCGCAGACTGGCTGCGCGACAGCCGCTCGGCTGAGGAATGG
Internal bar code:AGCTTAAATCCTGGGAGCTCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:2609
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:45
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:45

Suggested primers for confirmation by PCR

Suggested primer 1:CCACGGTCACGATACTCGAG
Suggested primer 2:CAAACGCGGCGTCTATCTTG