Insertion junction: CLIP2.005341_3

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.005341
Chromosome:chromosome 6
Location:6532763
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g800721 (1 of 1) K17582 - antigen KI-67 (MKI67) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTGTGGCTGGTGCAAGCGCTGGCCACCACCGCCGCTCCCTGCTGGAGGC
Internal bar code:TTTGGCTCATTACCAGAGAAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:562
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GACTATGCGCCAAACACGTC
Suggested primer 2:CACACCACCTAGTCCAGAGC