Insertion junction: CLIP2.005343_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.005343
Chromosome:chromosome 10
Location:2792319
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g438650 ADK2 Adenylate kinase; (1 of 1) 2.7.4.14 - UMP/CMP kinase / Uridine monophosphate-cytidine monophosphate phosphotransferase 3'UTR
Cre10.g801137 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACCAGCACAAGACGCTTTTGTCCACCTTGTATCTTACACCACCGCAACC
Internal bar code:GCCTCTTTATGGGTCCCCTCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:4094
LEAP-Seq percent confirming:74.7826
LEAP-Seq n confirming:86
LEAP-Seq n nonconfirming:29
LEAP-Seq n unique pos:115

Suggested primers for confirmation by PCR

Suggested primer 1:TCCACGCAAGCTCGTTAACT
Suggested primer 2:TGGCTAGTTCGTGTGTCCAC