| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.005350 |
| Chromosome: | chromosome 6 |
| Location: | 1459058 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g260250 | CEMA,CEM1,YCF10 | putative proton extrusion protein cemA, chloroplastic; (1 of 1) PTHR33650:SF1 - CEMA-LIKE PROTON EXTRUSION PROTEIN-LIKE PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGCTGGCACGCCTGTTCTACGGCCTGTCGGACATTGCCAAGGCCGTGT |
| Internal bar code: | TCTGGCCGTCACCGTAGTTATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 823 |
| LEAP-Seq percent confirming: | 81.8182 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACGTGCTGTTGGGACACT |
| Suggested primer 2: | TACTCCCCTAACCCCACTCG |