Insertion junction: CLIP2.005357_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.005357
Chromosome:chromosome 3
Location:5553889
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g185250 SSS2,SSII (1 of 1) IPR001296//IPR011835//IPR013534//IPR015421 - Glycosyl transferase, family 1 // Bacterial/plant glycogen synthase // Starch synthase, catalytic domain // Pyridoxal phosphate-dependent transferase, major region, subdomain 1; Soluble starch synthase II intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAAGGAATGGCCGGCCCACGTTTGCAGGCGTTCCTCGCGCGGGCGACGG
Internal bar code:TGTTTTTTTATTATACCTCGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:558
LEAP-Seq percent confirming:11.1111
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CACACTTTTTCGGCCATGCA
Suggested primer 2:TGCATTGAGCTTACCTGGCA