Insertion junction: CLIP2.005360_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.005360
Chromosome:chromosome 12
Location:7676768
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g555100 (1 of 726) IPR011009 - Protein kinase-like domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAAAGGGCGCGCTATTGTTCGCTATACCATAGTGATCACTGAACGTAGTC
Internal bar code:TACCCTAAATTTATCTGATTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:3949
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:77
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:77

Suggested primers for confirmation by PCR

Suggested primer 1:ACAGGAAGTCAGGTCGCAAG
Suggested primer 2:ACACTTCGTTGGAGCGTCAT