| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.005372 |
| Chromosome: | chromosome 6 |
| Location: | 7720447 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g302650 | RRM5 | (1 of 2) 2.1.1.166 - 23S rRNA (uridine(2552)-2'-O)-methyltransferase / Um2552 methyltransferase; Putative ribosomal RNA methyltransferase | 3'UTR |
| Cre06.g302700 | (1 of 1) PF00560//PF13516 - Leucine Rich Repeat (LRR_1) // Leucine Rich repeat (LRR_6) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTAGAGATGTGGGGACGTGGCACGCATGCCGGGAAAAGTGTCGGTCGT |
| Internal bar code: | TTTATTATTCAGTCTATGGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1122 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAACCCTGCAGCTTTCCAC |
| Suggested primer 2: | GGAGCATACGTACTCCGGTG |