Insertion junction: CLIP2.005376_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.005376
Chromosome:chromosome 1
Location:3700436
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g024050 (1 of 26) IPR013026//IPR019734 - Tetratricopeptide repeat-containing domain // Tetratricopeptide repeat CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGAAACGACTGGATCTGCAGATACAGATGCACGTAGCAAGGGGCATCCG
Internal bar code:ATCGCTGACTTACGTACGAGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:2468
LEAP-Seq percent confirming:84.6154
LEAP-Seq n confirming:44
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:52

Suggested primers for confirmation by PCR

Suggested primer 1:GGCCATTTCCTTCTCTGCCT
Suggested primer 2:GACGTGTTATGTGCGAGCAC