Insertion junction: CLIP2.005386_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.005386
Chromosome:chromosome 13
Location:1106602
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g569250 CGLD9 Conserved in the Green Lineage and Diatoms; (1 of 1) PF11255 - Protein of unknown function (DUF3054) (DUF3054) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCACAGGGGTCAGTGACGGGGACAGCGGGGCCGTGCCAGGTGCACCGGA
Internal bar code:CTAGGTTCGGAAACGACAAGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:2819
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:42
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:42

Suggested primers for confirmation by PCR

Suggested primer 1:GGGCTGGAGGTTGAGAGTTC
Suggested primer 2:GAGTCTATGCCAGATGCCCC