| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.005388 |
| Chromosome: | chromosome 11 |
| Location: | 3220836 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g476100 | OPR50,MCD1 | petD mRNA stabilization factor | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGACTTCCATGGATGCCGTGCTATACCGGTGGACACGTGAGGGTGGGA |
| Internal bar code: | CCTACTCAAATTGCTACCTTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1364 |
| LEAP-Seq percent confirming: | 70.7317 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGAGAGGAACTGCGTGTG |
| Suggested primer 2: | CCTCCCACGCTTTGTTTGTG |