Insertion junction: CLIP2.005413_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.005413
Chromosome:chromosome 16
Location:6026277
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCGGCCGGATAAACAAAATTGCACCCAGCCCAGGCACCGACGATCATGG
Internal bar code:ACCGTCGTGGATACAAAGTCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:2312
LEAP-Seq percent confirming:92.8571
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:CGTCTGATGCGCCGTATTTG
Suggested primer 2:ATCCTAGAGTCATGCGTGCG