| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.005433 |
| Chromosome: | chromosome 8 |
| Location: | 2445500 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g372000 | BAF3,CGLD6,CGLD11 | chloroplast ATPase CF1 assembly factor | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTCGAGGGGAGGAGGAAAGGGGAGGTGTGTGAGATGGGACAGGGTTCA |
| Internal bar code: | GAGATGCAAAGGCCATAGGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 509 |
| LEAP-Seq percent confirming: | 40.0 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAATCTGGAATTCAGGCGCG |
| Suggested primer 2: | CAAGTTCTGGATGCGCATCG |