| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.005450 |
| Chromosome: | chromosome 2 |
| Location: | 983896 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g079600 | TSF2 | (1 of 1) PTHR11538:SF41 - PHENYLALANINE--TRNA LIGASE, MITOCHONDRIAL; Phenylalanyl-tRNA synthetase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAGGGTCACGGGGTGAGCAGGACAGCCGTGCGCATGTTACTTTCTCACT |
| Internal bar code: | TATGACGCTTAGGTAACAGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1415 |
| LEAP-Seq percent confirming: | 17.4312 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 90 |
| LEAP-Seq n unique pos: | 109 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGACACAGTAGACAAGCCG |
| Suggested primer 2: | CTCACACTGTGCTTGTTGCC |