| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.005453 |
| Chromosome: | chromosome 2 |
| Location: | 6237075 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g387050 | FRK1 | (1 of 1) 2.7.1.45 - 2-dehydro-3-deoxygluconokinase / KDG kinase; Putative fructokinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGCCACAGGCGCAGCCGGTGACCCGGCACGGCTGGTCGGTACCCGGCGC |
| Internal bar code: | GGCTGCGTCAATGTGAGTCGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3582 |
| LEAP-Seq percent confirming: | 87.8049 |
| LEAP-Seq n confirming: | 108 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 123 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTCTTCACCATGCTCGTG |
| Suggested primer 2: | CGTCAGCCCTCTTCCTTTGT |