| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.005454 |
| Chromosome: | chromosome 12 |
| Location: | 3683385 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g513750 | GRX1 | Glutaredoxin, CPYC type, cytosolic; (1 of 2) K03676 - glutaredoxin 3 (grxC, GLRX, GLRX2) | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGTCCTTCAATACTCAACAACGAACGCTCGCATACTAAGCCACGGCAGC |
| Internal bar code: | GGTTCGGGCCTGGCCGACTACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4661 |
| LEAP-Seq percent confirming: | 92.9412 |
| LEAP-Seq n confirming: | 79 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 85 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGCCGGAATGCAATCATG |
| Suggested primer 2: | CTCACCGAAAGTTCCAGCCT |