| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.005466 |
| Chromosome: | chromosome 5 |
| Location: | 79041 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g242000 | CHLD,CHLD1 | (1 of 1) K03404 - magnesium chelatase subunit D (chlD, bchD); Magnesium chelatase subunit D, chloroplast precursor | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGTGGACGCCGGTCGACTGCCTGCAGGTGCGCATCGGCTATGCTATCCG |
| Internal bar code: | CACTGATACAAATTATCGGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5840 |
| LEAP-Seq percent confirming: | 86.4865 |
| LEAP-Seq n confirming: | 64 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 74 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGGCCAACCCCCATTAGTC |
| Suggested primer 2: | GCTACTAGGTGCCGAGTGAC |