| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.005470 |
| Chromosome: | chromosome 12 |
| Location: | 9194047 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g543303 | CGLD20,CGLD6 | conserved protein with COG5222 RING zinc finger domain; (1 of 1) PF08783 - DWNN domain (DWNN) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCCCGCCGCGGCACAGCCGCATAGAGTGGGTCATGCCAGGCAGCAGCC |
| Internal bar code: | TCGAAATCCTGGAGCCCCCTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1790 |
| LEAP-Seq percent confirming: | 66.6667 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCCCTCCAAACAGATCGG |
| Suggested primer 2: | CGGGGTACTCCAAAGGGAAC |