| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.005482 |
| Chromosome: | chromosome 5 |
| Location: | 2341538 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g238322 | TSW2 | (1 of 2) 6.1.1.2 - Tryptophan--tRNA ligase / Tryptophanyl-tRNA synthetase; Putative tryptophanyl-tRNA synthetase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGGTTAACTGGGGTACGCGGTGACGAGTCGCGGTGCGCGGGCCTCCAT |
| Internal bar code: | TTTGTATGCACGCAGTCTGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2706 |
| LEAP-Seq percent confirming: | 70.0 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTACGCGTCGGTCTACTC |
| Suggested primer 2: | CTGTGATCTTGGGTGCGAGT |