| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.005487 |
| Chromosome: | chromosome 16 |
| Location: | 5532877 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g682100 | TDR1 | (1 of 1) 1.1.1.206//1.1.1.236 - Tropinone reductase I / Tropinone reductase // Tropinone reductase II / Tropinone reductase; Tropine dehydrogenase/reductase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGCTGCGGCGATTCGGGCTGGTTGGTCGGACGTGCCTGGTCACCGGTG |
| Internal bar code: | TATGGTACGCATAATCCATTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 626 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGGCTCAACACTACCACG |
| Suggested primer 2: | CTGCGTTGTGAGGGTTTGTG |