Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | + |
Strain: | CLIP2.005498 |
Chromosome: | chromosome 7 |
Location: | 2193834 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre07.g327100 | (1 of 12) IPR000326 - Phosphatidic acid phosphatase type 2/haloperoxidase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACACACACACACACACACACTCGCATACACTGTCTTTGCACAACGTTTT |
Internal bar code: | CGGCCGTCTTGACTCAACGGGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 181 |
LEAP-Seq percent confirming: | 9.67742 |
LEAP-Seq n confirming: | 3 |
LEAP-Seq n nonconfirming: | 28 |
LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CAAGTCACACCCCTCTGTCC |
Suggested primer 2: | ATGGGGAGCAGGTTGGAATG |