Insertion junction: CLIP2.005498_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.005498
Chromosome:chromosome 7
Location:2193834
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g327100 (1 of 12) IPR000326 - Phosphatidic acid phosphatase type 2/haloperoxidase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACACACACACACACACACACTCGCATACACTGTCTTTGCACAACGTTTT
Internal bar code:CGGCCGTCTTGACTCAACGGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:181
LEAP-Seq percent confirming:9.67742
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:28
LEAP-Seq n unique pos:31

Suggested primers for confirmation by PCR

Suggested primer 1:CAAGTCACACCCCTCTGTCC
Suggested primer 2:ATGGGGAGCAGGTTGGAATG