| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.005516 |
| Chromosome: | chromosome 13 |
| Location: | 3883823 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g589650 | (1 of 1) PF08424 - NRDE-2, necessary for RNA interference (NRDE-2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCGAGGGACGTAGATTTACATGCATGCACGCTCTCAGCCTGCAGGCGT |
| Internal bar code: | ATCTGGCGACTATTGCTTGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1713 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTAAGAAAGCGGGCTGAGGG |
| Suggested primer 2: | CGGGAGTACAACACAGCAGT |