Insertion junction: CLIP2.005517_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.005517
Chromosome:chromosome 12
Location:5004963
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g525100 SMC6B (1 of 2) PTHR19306//PTHR19306:SF6 - STRUCTURAL MAINTENANCE OF CHROMOSOMES 5,6 SMC5, SMC6 // PROTEIN C23H4.6, ISOFORM A-RELATED; Structural Maintenance of Chromosomes protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTGCGTGAGGTTACAGTACTTGCACACGTCGCCCCACCGCTGCCCCGCA
Internal bar code:GATCAGTTCTGGTCTTCTCGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:749
LEAP-Seq percent confirming:90.9091
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:GGGTTGGGTTAGAGAAGGGC
Suggested primer 2:CAGTACCCACCACTGAGCTG