| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.005546 |
| Chromosome: | chromosome 17 |
| Location: | 3117915 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g720550 | (1 of 1) PTHR34202:SF1 - UPF0548 PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTGCGGTGACTCAGATGCACGCCAATACAACAACGCGCAAAGCATGTT |
| Internal bar code: | ATCATCTGACCAAGAGCGCTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3189 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 74 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 74 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGTAGGGAAGACCAGGGAG |
| Suggested primer 2: | CTAGCACCGCAATTCATGGC |