| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.005560 |
| Chromosome: | chromosome 13 |
| Location: | 1701259 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g574200 | PAP2 | (1 of 3) K03514 - non-canonical poly(A) RNA polymerase PAPD5/7 (PAPD5_7, TRF4); Class-II RNA nucleotidyl transferase 2 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCTCATGTTGTTGGCGCGCACAGATGCCAGAGGATGACCTGTTGGTCGG |
| Internal bar code: | TGGGTTTCGTAAACCAGTTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4892 |
| LEAP-Seq percent confirming: | 93.5484 |
| LEAP-Seq n confirming: | 87 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 93 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACATACTGCAGCCGAATGC |
| Suggested primer 2: | CGCTGCTTTCATTCTCGCTC |