| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.005567 |
| Chromosome: | chromosome 15 |
| Location: | 300987 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g635100 | (1 of 2) IPR000104//IPR010402 - Antifreeze protein, type I // CCT domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGCGCGCTTTTGATGAGGCGCCTGGCTTCGCGGTTAGGGTTGTTTGAA |
| Internal bar code: | TTATATTCAGAGACGGCTAATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 952 |
| LEAP-Seq percent confirming: | 2.08333 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 47 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTAAAGGGCCTCGAACAGC |
| Suggested primer 2: | CATGATGCGCCCCTTTCATG |