| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.005574 |
| Chromosome: | chromosome 10 |
| Location: | 3461569 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g444500 | (1 of 7) 2.8.1.1 - Thiosulfate sulfurtransferase / Thiosulfate thiotransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGAGGGGAGGGAGATCACCGTCAAGGCGATCAGTGGCGCAAGCGCTAC |
| Internal bar code: | TTTTTTCGAATCCATAGGATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3346 |
| LEAP-Seq percent confirming: | 71.5385 |
| LEAP-Seq n confirming: | 93 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 130 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCAACTGACTCAGACGTCA |
| Suggested primer 2: | GTGGCTGCAGGACAACCTAT |