Insertion junction: CLIP2.005581_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.005581
Chromosome:chromosome 3
Location:8789224
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g207153 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACACACGCACACATACACACACACGCACACACACATACACACACATGTT
Internal bar code:CGCGTAAGTATCGTCCGTGTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:391
LEAP-Seq percent confirming:44.4444
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:GGGAGCTTGGATGCACTCTT
Suggested primer 2:CAACATCGATCAGCAGTGCG