| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.005586 |
| Chromosome: | chromosome 10 |
| Location: | 1941849 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g431850 | PGM18 | Phosphoglycerate mutase family protein; (1 of 1) PTHR23029//PTHR23029:SF49 - PHOSPHOGLYCERATE MUTASE // PHOSPHOHISTIDINE PHOSPHATASE SIXA | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAAGGGGGCGGCAAGACGCTGACTAGTACCCCCGCTCAGAGCGGGGCAG |
| Internal bar code: | GGATGCAGAGGAGGAGGATATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2924 |
| LEAP-Seq percent confirming: | 91.0714 |
| LEAP-Seq n confirming: | 51 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 56 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCGTAATAGTGCGCGTCC |
| Suggested primer 2: | TATGCACGTTGACCACTGCT |