| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.005587 |
| Chromosome: | chromosome 12 |
| Location: | 8348887 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g550800 | REV1 | (1 of 1) K03515 - DNA repair protein REV1 (REV1); Deoxycytidyl transferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCAACAGCCTCCTCCTGCTCCTGGAAAGGCTCCGACTCGGGCTCCCCA |
| Internal bar code: | CTTTCTATGGCCAAAACGTTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 954 |
| LEAP-Seq percent confirming: | 22.2222 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGCCCTCTTTCACTGTCAC |
| Suggested primer 2: | CCCCACTCCACTCCATTTCC |