| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.005612 |
| Chromosome: | chromosome 16 |
| Location: | 1169302 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g650400 | CPL20 | Hypothetical zinc-dependent DNA-binding protein; (1 of 1) IPR000104//IPR006734 - Antifreeze protein, type I // Protein of unknown function DUF597 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCCCCTAGACGAAGCCACAGCGACACACGCATCTACACAAAACCCAAC |
| Internal bar code: | CAGTGGCGCGTTTAAGGTCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2924 |
| LEAP-Seq percent confirming: | 96.7742 |
| LEAP-Seq n confirming: | 90 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 93 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTACGTGAGCATTGTGCCT |
| Suggested primer 2: | AATGTATGTCTGTGCCGGCA |