| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.005616 |
| Chromosome: | chromosome 12 |
| Location: | 8507464 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g549500 | (1 of 2) 1.5.4.1 - Pyrimidodiazepine synthase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCACGTAGTGCACCGGGCACTGGGCGTCCACCCTGCGGGCGGCGGAGCGG |
| Internal bar code: | ACCCCGAATTCTTGACCTTATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2470 |
| LEAP-Seq percent confirming: | 77.2727 |
| LEAP-Seq n confirming: | 51 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 66 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACGGTTTATGCGCGCATA |
| Suggested primer 2: | CCGCACTCTCCATGTCAAGT |