| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.005617 |
| Chromosome: | chromosome 17 |
| Location: | 3200590 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g721350 | GST13 | Glutathione S-transferase; (1 of 6) K00799 - glutathione S-transferase (GST, gst) | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGGACGGTGTCACCTCCATCCGCCTATCACGCCTCGATTGCGTGTACG |
| Internal bar code: | CTGCCCAGATTAAGCTGTCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3330 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 52 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 52 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTTACAAACCGCCAAGCCG |
| Suggested primer 2: | GATCACGCTGAGACCTAGCC |