Insertion junction: CLIP2.005621_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.005621
Chromosome:chromosome 12
Location:3138506
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g500650 RNB2 (1 of 1) PTHR23355//PTHR23355:SF39 - RIBONUCLEASE // SUBFAMILY NOT NAMED; chloroplast RNase II CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTGCTGGCGGCCGGCACGCGGCGCAGCGCCCCCCGGCGCCCTTCCGCTC
Internal bar code:TGAGTCGTCCTTGCGTTCCGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:1650
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:TAACAACGCACCTCCCCTTC
Suggested primer 2:CACATAAGTTACGTGCGCGG