Insertion junction: CLIP2.005623_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.005623
Chromosome:chromosome 2
Location:3473278
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g098950 SYP72 Qc-SNARE protein, SYP7-family; (1 of 2) K08506 - syntaxin of plants SYP7 (SYP7) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTGATGTGTAGACACACGCGCATTAATAATGGCGGGGCTCTGAAGCGTC
Internal bar code:CAGCCTTCATAATGCCTGATGC

Confirmation - LEAP-Seq

LEAP-Seq distance:789
LEAP-Seq percent confirming:94.1176
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:AAACAAAGTGCCAGACCCCA
Suggested primer 2:GTTCTGTTCCAAACGTGCCC