Insertion junction: CLIP2.005656_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.005656
Chromosome:chromosome 12
Location:6026171
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g534600 TOM20 (1 of 1) PTHR32409:SF3 - MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM20-1-RELATED; Mitochondrial translocase of outer membrane, 20 kDa intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCATCCAACACGCCCAACGAGCCAACGACTGCTGCGGCATCCAACCCAC
Internal bar code:TACTGATGCATGCCGGTCCATT

Confirmation - LEAP-Seq

LEAP-Seq distance:2000
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:17
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:CTCTCCTCCTCCTCCTGCTT
Suggested primer 2:TTGGCTGGGTGTTCAGGTTT