| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.005659 |
| Chromosome: | chromosome 3 |
| Location: | 4948205 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g179200 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCAGCGGTGTTGATTATAACAGCAACCTGATATGACGGGTAGATGCTAC |
| Internal bar code: | CAGTGGAACTCACTGTGGTAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 884 |
| LEAP-Seq percent confirming: | 87.0968 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGGCGTTAGAGCATAGTC |
| Suggested primer 2: | ACGGAGTGAGTCATTCGTCG |