| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.005661 |
| Chromosome: | chromosome 1 |
| Location: | 7744626 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g054500 | (1 of 1) 1.6.1.2 - NAD(P)(+) transhydrogenase (Re/Si-specific) / Transhydrogenase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGGCTCTATACAAATTGTGTCTTCTTTCGTGTGCGTGCGAGTATAACG |
| Internal bar code: | ATGCTCGAGTACGTTTGCGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4068 |
| LEAP-Seq percent confirming: | 93.75 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTGGTGCCAAACTTGAGC |
| Suggested primer 2: | GATGGTCTTGAAGCCCTGCT |