| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.005678 |
| Chromosome: | chromosome 12 |
| Location: | 481175 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g494550 | RNP10 | RNA binding protein; (1 of 3) K14947 - epithelial splicing regulatory protein 1/2 (ESRP1_2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGAGGGCGTCAGCTTCATCATGCACGCGGACGGGCGGCCCACCGGCAT |
| Internal bar code: | CAAATTGGATTTTCGTGCGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1243 |
| LEAP-Seq percent confirming: | 64.2857 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATGCAAGATGACTCCGCA |
| Suggested primer 2: | GACCCTCGATTCGGACATCC |