Insertion junction: CLIP2.005680_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.005680
Chromosome:chromosome 6
Location:4902165
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g280650 CGL59,Y3IP1 Chloroplast Ycf3 interacting protein involved in Photosystem I biogensis 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTCATATTCTAATAATAAATCTAACACATTCAATCTATTGGATATCACT
Internal bar code:ATAAGGTGCAAATACTCTGACT

Confirmation - LEAP-Seq

LEAP-Seq distance:2531
LEAP-Seq percent confirming:97.0588
LEAP-Seq n confirming:33
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:34

Suggested primers for confirmation by PCR

Suggested primer 1:GAGGTGTGTGAGGGTCTTCG
Suggested primer 2:CCTCAAAAATGTCCGCGCAA