| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.005687 |
| Chromosome: | chromosome 12 |
| Location: | 9200296 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g543100 | (1 of 1) K03256 - tRNA (adenine-N(1)-)-methyltransferase non-catalytic subunit (TRM6, GCD10) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCGAGGTCACCGCCGACGGCAAGAGCTTGCAGCGGGTTCTCCTGTGAG |
| Internal bar code: | ATGCGTATCTAAGCTTACAAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 890 |
| LEAP-Seq percent confirming: | 77.2727 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCACCTCCGAGATCATGC |
| Suggested primer 2: | AGGGCAGGTTGCTGTAAGAC |