| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.005692 |
| Chromosome: | chromosome 2 |
| Location: | 2087683 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g088850 | RFS2 | Riboflavin synthase; (1 of 1) 2.5.1.78 - 6,7-dimethyl-8-ribityllumazine synthase / Lumazine synthase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTGATAACACGCGCGCGTCCTCCAGGTCCGGCTGGCTGCCATTTACCA |
| Internal bar code: | ATGCAGGTGACTGTTTCCAAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 676 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCCATTGTCATCATGCAT |
| Suggested primer 2: | CCCCAATTCCTGCCAGACAT |