| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.005692 |
| Chromosome: | chromosome 2 |
| Location: | 2087684 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g088850 | RFS2 | Riboflavin synthase; (1 of 1) 2.5.1.78 - 6,7-dimethyl-8-ribityllumazine synthase / Lumazine synthase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCATGCCGGAGCGGGCGTCATGTAGAGCCACTGGTGTGAAAATCCTGAC |
| Internal bar code: | TTAGTGTTTCAGATGAATGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1039 |
| LEAP-Seq percent confirming: | 30.7692 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCAATTCCTGCCAGACAT |
| Suggested primer 2: | GCGCCATTGTCATCATGCAT |