Insertion junction: CLIP2.005693_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.005693
Chromosome:chromosome 2
Location:2428133
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g091400 CLPB2 (1 of 1) IPR001270//IPR003593//IPR003959//IPR024064//IPR027417 - ClpA/B family // AAA+ ATPase domain // ATPase, AAA-type, core // FdhE-like // P-loop containing nucleoside triphosphate hydrolase; ClpB chaperone, Hsp100 family intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGGCTGAAAGCCGCAAAGCCATGCAACCACGCTGCCAACGGTTTGCGCC
Internal bar code:ACGTTTGCGAGCGCTCGTCGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:3257
LEAP-Seq percent confirming:94.9153
LEAP-Seq n confirming:56
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:59

Suggested primers for confirmation by PCR

Suggested primer 1:TTGAAACAGGCTGTGGGGTT
Suggested primer 2:GTATGTATGTGCGCGGAGGA