| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.005694 |
| Chromosome: | chromosome 4 |
| Location: | 529141 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g217932 | PRPL35 | Chloroplast ribosomal protein L35; (1 of 1) K02916 - large subunit ribosomal protein L35 (RP-L35, MRPL35, rpmI) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGTGTGATTTCCACGTGCTGCTGGCAGATCTGGCAGGTTGGTGTGCTT |
| Internal bar code: | AACAATCGAAGGTTCGGCCTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2711 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 65 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 65 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATCCAGGACGTTATGCGCC |
| Suggested primer 2: | ATAAAGGAGCTCGGGAGGGT |