Insertion junction: CLIP2.005696_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.005696
Chromosome:chromosome 12
Location:5764591
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g532600 CGL44 RabGAP/TBC Domain Protein; (1 of 14) PF00566 - Rab-GTPase-TBC domain (RabGAP-TBC) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTGCAAGGGGTGGTCGCCGGTTGAGACATGGCCCGCACCTTGACCATGA
Internal bar code:GAATGTCATTGACGGTGATATT

Confirmation - LEAP-Seq

LEAP-Seq distance:3174
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:30
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:30

Suggested primers for confirmation by PCR

Suggested primer 1:CTGAACCCATCCAACTGGCT
Suggested primer 2:GTTCTGCAGCCGACACAATG