Insertion junction: CLIP2.005701_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.005701
Chromosome:chromosome 16
Location:5531514
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g682100 TDR1 (1 of 1) 1.1.1.206//1.1.1.236 - Tropinone reductase I / Tropinone reductase // Tropinone reductase II / Tropinone reductase; Tropine dehydrogenase/reductase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCACAGCCCGGGCGGACAACGGCAAGCAACGTGCCTTCGCACATGCCGAC
Internal bar code:AGACCTCAACCTTCAATTTGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:1494
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:TGCAGCCCAGGTTTTCGTAT
Suggested primer 2:ACGTGTGATAGGGCTGTGTG