| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.005701 |
| Chromosome: | chromosome 16 |
| Location: | 5531519 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g682100 | TDR1 | (1 of 1) 1.1.1.206//1.1.1.236 - Tropinone reductase I / Tropinone reductase // Tropinone reductase II / Tropinone reductase; Tropine dehydrogenase/reductase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACCCTGCCTGCCTGCCTGCCTGCCTGCCCCTCACCCACCTCCTCCGAG |
| Internal bar code: | AGACCTCAACCTTCAATTTGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 451 |
| LEAP-Seq percent confirming: | 83.3333 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTGTGATAGGGCTGTGTG |
| Suggested primer 2: | TGCAGCCCAGGTTTTCGTAT |