| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.005715 |
| Chromosome: | chromosome 1 |
| Location: | 5534648 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g039450 | (1 of 2) PTHR34548//PTHR34548:SF2 - FAMILY NOT NAMED // PROTEIN TIC 21, CHLOROPLASTIC | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCAGTCGCACAAGCGGCCCCCGAACTGAAAGTGACCCTTCTGTCCTCCA |
| Internal bar code: | GAACATGGACGGGTTGTTGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1840 |
| LEAP-Seq percent confirming: | 93.5484 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGATGTGCCTGGGTTTTGC |
| Suggested primer 2: | CGATGGCGGTGATGTTGAAC |