| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.005725 |
| Chromosome: | chromosome 1 |
| Location: | 120435 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g000900 | CPLD20 | Conserved in the Plant Lineage and Diatoms; (1 of 2) PF07082 - Protein of unknown function (DUF1350) (DUF1350) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCAAGCATGCGCTTGTTGTTGCGAGCGAGGGCCCTCCATGCAAGTGCC |
| Internal bar code: | AATGCCGTCCCGAAAGTTTCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3050 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 89 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 89 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCCGATAAGGATGACGTC |
| Suggested primer 2: | GTATAGGCCCGGAGACTTGC |